金英黄归液的抑菌作用分析

doi:10.11843/j.issn.0366-6964.2013.09.023

Abstract

Abstract:

The aim of this study was to study antibacterial effects of Jin-Ying-Huang-Gui Injection (JYHGI), a Chinese herbal formula, on Staphylococcus aureus (S. aureus)．JYHGI was prepared by routine method. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of S. aureus were detected with tube double dilution method in vitro. The mice model was prepared by vena caudalis injection of S. aureus to carry antibacterial experiment in vivo. In addition, we also studied the influence of JYHGI on ultrastructure and the specific activity of succinate dehydrogenase (SDH) of S. aureus. Antibacterial activity showed that the MIC and MBC of JYHGI to S. aureus were 31.2, 62.5 mg·mL^－1, respectively. Mortality of mice in JYHGI could significantly decrease mice mortality caused by S. aureus (P<0.05). The JYHGI treated cell wall of S. aureus were covered with a large number of floc, the cells seemed to be collapsed-like, some cell wall even ruptured. The SDH specific activity of JYHGI group were significantly lower than that of control group (P<0.01). It is concluded that the inhibition mechanism is that JYHGI could damage the cell wall and inhibit the enzyme system of S.aureus.

CLC Number:

S853.74

YANG Hang, ZHANG Xiao-yi, WANG Lu. Antibacterial Effects of Jin-Ying-Huang-Gui Injection to Staphylococcus aureus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(9): 1494-1498.

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References

［1］GONZÁLEZ R N, WILSON D J, SICKLES S A, et al. Outbreaks of clinical mastitis caused by Trichosporon beigelii in dairy herds［J］. J Am Vet Med Assoc, 2001, 218(2):238-242.

［2］陈玉霞, 林峰. 3种奶牛乳房炎主要病原菌PCR检测方法的建立［J］. 中国农学通报, 2012, 28(17):105-108.

［3］郭庆, 王鲁, 朱伟, 等. 中药透皮剂的制备及其对奶牛隐性乳房炎临床疗效［J］. 中国兽医杂志, 2010, 46(5):45-46.

［4］WANG L U, HE C L, HE B K, et al. Effects of Jin-Ying-Tang on Staphylococcus aureus-induced mastitis in rabbit［J］. Immunopharmacol Immunotoxicol, 2012, 34(5):786-793.

［5］李仪奎. 中药药理实验方法学［M］. 上海: 上海科学出版社, 1991: 1415-1419.

［6］王清, 朱萱萱, 张赤兵,等. 金银花提取物抗菌作用的实验研究［J］. 中国医药导刊, 2008, 10(9):1428-1430.

［7］王储炎. 鹿蹄草提取物的抑菌作用和应用研究［D］. 重庆: 西南大学, 2007: 22-24.

［8］徐明生, 陈锦屏, 上官新晨. 鱼精蛋白对黑曲霉细胞内的玻拍酸脱氢酶和苹果酸脱氢酶的影响［J］. 食品科学, 2005, 26(4):48-51.

［9］路保通, 张鸯, 胡新安,等. 蒲公英有效成分提取及其抑菌活性作用［J］. 中国兽医杂志, 2008, 44(5):45-46.

［10］顾仁勇, 李佑稷, 傅伟昌. 连翘精油抑菌及抗氧化作用研究［J］. 现代食品科技, 2008, 24(2):120-122.

［11］石姗姗. 大豆异黄酮抑菌活性的研究及抑菌机制的初步探讨［D］. 大连: 辽宁师范大学, 2006.

［12］覃容贵, 李淑芳, 罗忠圣. 九头狮子草对细菌超微形态结构的影响［J］. 中国实验方剂学, 2005, 11(1):46-48.

［13］李文娟. 瑞香狼毒抑菌活性及其作用机制的初步研究［D］. 成都: 四川大学, 2006.

［14］SHIOTA S, SHIMIZU M, SUGIYAMA J, et al. Mechanisms of action of corilagin and tellimagrandin I that remarkably potentiate the activity of beta-lactams against methicillin-resistant Staphylococcus aureus［J］. Microbiol Immunol, 2004, 48(1):67-73.

［15］陈杖榴. 兽医药理学［M］. 2版. 北京: 中国农业出版社, 2002.

［16］辛明秀, 周培谨. 冷活性琥珀酸脱氢酶的特性［J］. 北京师范大学学报(自然科学版), 2004, 40(1):108-111.

［17］YANKOVSKAYA V, HORSEFIELD R, TRNROTH S, et al. Architecture of succinate dehydrogenase and reactive oxygen species generation［J］. Science, 2003, 299(5607):700-704.

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Antibacterial Effects of Jin-Ying-Huang-Gui Injection to Staphylococcus aureus

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